In a particular species of mammal black hair (B) is dominant to green hair (b) and red eyes (R) are dominant to white eyes (r). If a BbRr individual is mated with a bbrrindividual the expected phenotypic ratio of the offspring is 1 black-red : 1 black-white: 1 green-red : 1 green-white. However, when you mate these individuals you find that the phenotypic ratio of the offspring is 6 black-red :1 black-white : 1 green-red : 6 green-white. What could account for this difference?

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#Question id: 13091

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

Which colony’s plasmid do you actually want to use for your studies?

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#Question id: 19695

#SCPH06 I Botany

Which of the following leads are used to record ECG by using two active electrodes?

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#Question id: 22949

#SCPH06 I Botany

How CONSTANS gene and its encoded protein involved in translating day length into a signal that converts a vegetative shoot meristem into a floral meristem?

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#Question id: 1029

#SCPH06 I Botany

The structures used for motility in both eukaryotes and prokaryotes are

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#Question id: 4594

#SCPH06 I Botany

Which one of the following observations did Darwin first make during his discovery of evolution?