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TLS Online TPP Program

#Question id: 4919

Genes for cytoplasmic male sterility in plants are generally located in

#SCPH28 | Zoology

  1. Mitochondrial genome

  2. Cytosol

  3. Chloroplast genome

  4. Nuclear genome

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TLS Online TPP Program

#Question id: 15657

#SCPH01 Biochemistry

You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium.
You next construct a MelA– MelB– double mutant, which gives the following behavior:
 
Which of your two models is consistent with this new data?
a) the MelA- MelB- double mutant is identical to the MelB- mutant
b) MelA- is epistatic to MelB-
c) MelB- is epistatic to MelA-
d) the MelA- MelB- double mutant is identical to the MelA- mutant
Which of the following is the correct prediction about the double mutants?
TLS Online TPP Program

#Question id: 15562

#SCPH28 | Zoology

Deletion of which gene not only was accompanied by the abolition of antibiotic formation but also severely impaired growth on agar media.
TLS Online TPP Program

#Question id: 2986

#SCPH06 I Botany

During ubiquitin-mediated protein degradation in various events of cell cycle, ubiquitin-protein ligases ubiquitinylate substrate proteins, marking them for degradation by proteasomes. Cyclins are degraded through the action of two different ubiquitin-protein ligases, SCF and the anaphase-promoting complex or cyclosome. Following statements are regarding to SCF and APC/C.

A. SCF controls the G1–S phase transition by degrading G1/S phase cyclins and, CDK inhibitory proteins.

B. APC/C degrades S phase and mitotic cyclins, thereby promoting the exit from mitosis.

C. SCF and APC/C are multi-subunit ubiquitin-protein ligases that belong to the RING finger family of ubiquitin protein ligases.

D. SCF and APC/C belong to the different ubiquitin-protein ligase family, their regulation is same.

E. SCF recognizes its substrates only when they are phosphorylated.

F. SCF and APC/C both recognizes its substrates only when they are phosphorylated.

Which of the following is incorrect?
TLS Online TPP Program

#Question id: 14674

#SCPH05 I Biotechnology

How many moles of glucose are in 19.1g of glucose?
TLS Online TPP Program

#Question id: 13076

#SCPH06 I Botany

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.