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TLS Online TPP Program

#Question id: 8692

Which of the following statements is accurate with regard to a phylogeny, as represented by a phylogenetic tree?

#SCPH28 | Zoology
  1. Descendant groups (branches) from the same node likely share many derived characters.
  2. A monophyletic group can be properly based on convergent features.
  3. The ancestral group often has all the derived characters of the descendant species.
  4. Shared ancestral characters are excellent traits to use in developing a phylogeny.
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TLS Online TPP Program

#Question id: 1582

#SCPH01 Biochemistry

The EBV antigen fragments will be presented by the virus-infected cells along with which of the following?
TLS Online TPP Program

#Question id: 14116

#SCPH05 I Biotechnology

Which of the following statements are CORRECT when a protein sequence database is searched using the BLAST algorithm? 
P. A larger E-value indicates higher sequence similarity 
Q. E-value < 10^-10 indicates sequence homology 
R. A higher BLAST score indicates higher sequence similarity 
S. E-value > 10^10 indicates sequence homology 
TLS Online TPP Program

#Question id: 14091

#I Life Science/ Life Sciences Group – I-V

An example of a composite database:
TLS Online TPP Program

#Question id: 13089

#SCPH28 | Zoology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
                              
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
 
What is the Colony 2’s plasmid is;
TLS Online TPP Program

#Question id: 3631

#SCPH28 | Zoology

Six different mutations 1 to 6, from the following results of functional allelism tests whereby the mutants were crossed with each other, determine location of mutant gene.