What is another hypothesis regarding the formation of polymers such as proteins and nucleic acids?

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#Question id: 8944

#SCPH06 I Botany

Which two groups are most clearly represented in the Ediacaran fauna?

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#Question id: 20847

#SCPH01 Biochemistry

In groundwater hydrocarbon spill which metal reduction was the major electron accepting process?

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#Question id: 2617

#SCPH01 Biochemistry

According role of regulatory proteins, RNA polymerase and one or more promoter elements, mostly three levels of transcription are there, choose correct one

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#Question id: 5256

#SCPH28 | Zoology

If the experimental population of E. coli lacks an F factor or F plasmid, and if bacteriophage are excluded from the bacterial cultures, then which of these is a means by which beneficial mutations might be transmitted horizontally to other E. coli cells?

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#Question id: 13091

#SCPH28 | Zoology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

Which colony’s plasmid do you actually want to use for your studies?