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TLS Online TPP Program
#Question id: 13054
#SCPH28 | Zoology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?
TLS Online TPP Program
#Question id: 4947
#SCPH06 I Botany
The first genes on Earth were probably
TLS Online TPP Program
#Question id: 20105
#SCPH05 I Biotechnology
Identify the correct statement regarding a spontaneous process :
TLS Online TPP Program
#Question id: 10978
#SCPH06 I Botany
Which of the following events occurs at the end of the period of ventricular ejection?
TLS Online TPP Program
#Question id: 563
#I Life Science/ Life Sciences Group – I-V
Both water and glucose share an —OH that can serve as a substrate for a reaction with the terminal phosphate of ATP catalyzed by hexokinase. Glucose, however, is about a million times more reactive as a substrate than water. The best explanation is that: