A novel technique for strain improvement combining the advantage of multi-parental crossing allowed by DNA shuffling with the recombination of entire genomes.

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#Question id: 2970

#SCPH28 | Zoology

Describe how Cdc14 triggers the exit from mitosis in the budding yeast, S. cerevisiae.

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#Question id: 11452

#SCPH28 | Zoology

A given photon of light may trigger an action potential with thousands of times more energy because the signal strength is amplified by ________.

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#Question id: 8933

#SCPH06 I Botany

At which developmental stage should one be able to first distinguish a diploblastic embryo from a triploblastic embryo?

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#Question id: 14774

#SCPH06 I Botany

A problem in mathematics is given to three students Dayanand, Ramesh and Naresh whose chances of solving it are 1/2, 1/3, 1/4 respectively. The probability that the problem is solved is

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#Question id: 13055

#SCPH06 I Botany

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?