TLS Online TPP Program
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TLS Online TPP Program
#Question id: 13056
#SCPH28 | Zoology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following is true for traditional and real time PCR?
TLS Online TPP Program
#Question id: 12750
#SCPH28 | Zoology
Bacterial culture was treated with SDS and NAOH then centrifuged, supernatant was decanted and treated with potassium acetate and centrifuged to get a pellet. What is the composition of the pellet?
TLS Online TPP Program
#Question id: 13066
#SCPH05 I Biotechnology
Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following is a codominant marker?
TLS Online TPP Program
#Question id: 1418
#SCPH06 I Botany
Apoptosis can be induced by a mitochondrial protein being released into the cytosol. This protein binds directly to what in the cytosol?
TLS Online TPP Program
#Question id: 2644
#SCPH05 I Biotechnology
Which of these items have a positive impact on PEDCBA transcription?