#Question id: 1590
#I Life Science/ Life Sciences Group – I-V
An epitope associates with which part of an antibody?
#Question id: 643
#SCPH05 I Biotechnology
The hydrolysis of lactose is catalysed by b-galactosidase as given below
Which of the following could be correct mechanism for this hydrolysis reaction?
A. Nucleophilic attack by the carboxylate group of Glu/Asp on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.
B. Electrophilic attack by the carboxylate group of Glu/Asp on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.
C. Nucleophilic attack by the carboxylate group of Lys/Arg/His or bivalent cation on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.
#Question id: 4185
#SCPH28 | Zoology
Which of the following is mismatched?
#Question id: 1106
#SCPH01 Biochemistry
Protein kinase is an enzyme that functions in which of the following ways?
#Question id: 573
#SCPH01 Biochemistry
How is trypsinogen converted to trypsin?