According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

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#Question id: 4586

#SCPH28 | Zoology

 What are adaptations?

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#Question id: 5031

#SCPH28 | Zoology

Which era is known as the ʺage of reptilesʺ?

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#Question id: 19684

#SCPH01 Biochemistry

Which of the following is used to measure the electrical activity of the heart?

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#Question id: 10520

#SCPH06 I Botany

Which one of the following fungus causes infected rice shoots to grow much faster relative to uninfected plants and responsible for the “foolish seedling disease” of rice?

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#Question id: 12943

#SCPH01 Biochemistry

Choose the correct order for the steps involve in Insitu hybridization.

1. Cell permeaabilisation

2. Detection of hybridised probe radioactive.

3. Wash excess probe from section

4. Cell fixation