Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order

i. incubate with antibody-enzyme complex that binds primary antibody

ii. coat surface with antigen, block unoccupied sites with nonspecific protein

iii. add substrate, formation of colored product indicates presence of specific antigen

iv. incubate with primary antibody against specific antigen

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#Question id: 15734

#SCPH05 I Biotechnology

In seed germination or seed culture process growth regulators are usually not necessary because, of

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#Question id: 546

#SCPH05 I Biotechnology

Enzymes are potent catalysts because they:

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#Question id: 45

#SCPH28 | Zoology

What is the standard free-energy change, ΔG°, under physiological conditions (E. coli grows in the human gut, at 37 °C) for the following reaction? (Keq = 7.8 × 10^2)

Glucose + ATP → glucose 6-phosphate + ADP

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#Question id: 28033

#Research Methodology

The basis on which assumptions are formulated:

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#Question id: 14349

#SCPH05 I Biotechnology

An aerated bioreactor will increase oxygen transfer rates with the addition of detergents because it