Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 19766

#SCPH05 I Biotechnology

Which of the following molecule possesses linear structure ?

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#Question id: 19348

#SCPH28 | Zoology

Nuclear gene rbcs encodes the smaller subunit of

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#Question id: 18688

#I Life Science/ Life Sciences Group – I-V

Match the items in Group 1 with an appropriate description in Group 2 

Group I                                      Group 2 

P. GOR method                           1. Protein structure visualization 

Q. Genome sequencing           2. Sanger method

R. ESTs                                         3. Protein secondary structure prediction

S. RasMol                                    4. gene expressed as cDNA

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#Question id: 2643

#SCPH28 | Zoology

 If in an experiment you mutate the two trp codons in the attenuator to ala codon what will be the impact on repression?

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#Question id: 5262

#SCPH28 | Zoology

Which of the following characteristics is not true of a plasmid?