Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

#Question id: 9272

#SCPH05 I Biotechnology

#Question id: 9272

#SCPH06 I Botany

#Question id: 9273

#I Life Science/ Life Sciences Group – I-V

In the higher excited state, chlorophyll is extremely unstable; it rapidly gives up some of its energy to the surroundings as heat, and enters the lowest excited state, the excited chlorophyll has four alternative pathways for disposing of its available energy: fluorescence, Heat loss, energy transfer, photochemistry; all process takes place in which wavelength,

#Question id: 9273

#SCPH05 I Biotechnology

In the higher excited state, chlorophyll is extremely unstable; it rapidly gives up some of its energy to the surroundings as heat, and enters the lowest excited state, the excited chlorophyll has four alternative pathways for disposing of its available energy: fluorescence, Heat loss, energy transfer, photochemistry; all process takes place in which wavelength,

#Question id: 9273

#SCPH06 I Botany

In the higher excited state, chlorophyll is extremely unstable; it rapidly gives up some of its energy to the surroundings as heat, and enters the lowest excited state, the excited chlorophyll has four alternative pathways for disposing of its available energy: fluorescence, Heat loss, energy transfer, photochemistry; all process takes place in which wavelength,

#Question id: 9275

#I Life Science/ Life Sciences Group – I-V

The colour of carrots, is due to;