Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

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#Question id: 19769

#SCPH05 I Biotechnology

Which of the following is not a characteristic of Planck’s quantum theory of radiations?

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#Question id: 2304

#I Life Science/ Life Sciences Group – I-V

Which of the following is generally absent from animal cells?

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#Question id: 1602

#SCPH05 I Biotechnology

Following are some of the characteristics of MHC 1 and MHC 2 both , except 

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#Question id: 7702

#I Life Science/ Life Sciences Group – I-V

If organisms A, B, and C belong to the same class but to different orders and if organisms C, D, and E belong to the same order but to different families, which of the following pairs of organisms would be expected to show the greatest degree of structural homology?

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#Question id: 11671

#I Life Science/ Life Sciences Group – I-V

Which process in the nephron is least selective?