Practice Questions

TLS Online TPP Program

#Question id: 13056

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following is true for traditional and real time PCR?

#SCPH28 | Zoology

Both the techniques allow detection of the product in early stages
Real time PCR can detect the product in early stages as compared to traditional PCR
Both systems require different primers
Both techniques are end point product determination methods