Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 11454

#SCPH28 | Zoology

The structure of the mammalian middle ear is adapted to convert ________.

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#Question id: 11913

#SCPH28 | Zoology

During the rate of succession several factors would be observe at the pioneer as well as climax community;

Match the community stages with its given characters;

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#Question id: 9223

#SCPH28 | Zoology

Managing southeastern forests specifically for the red-cockaded woodpecker ________.

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#Question id: 5071

#SCPH06 I Botany

Defect in hedgehog signaling cause, basal cell nevus syndrome, a rare autosomal dominant condition characterized by both developmental anomalies (fused fingers; rib and facial abnormalities) and multiple malignant tumors. This condition created due to mutation in

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#Question id: 87

#SCPH28 | Zoology

The compounds α-D-fructofuranose and β-D-fructofuranose are ________.