Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

#Question id: 10970

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A mother of blood type A+ who has always been perfectly healthy just delivered her second child. The father is of blood group O−. Knowing that the child is of blood group O− (O, Rh−), what would you expect to find in this child?

#Question id: 10971

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A pregnant woman comes in for a visit. She is AB Rh− and her husband is A Rh+. This is her first child. What should be done at this time?

#Question id: 10972

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What is the proper pathway for the extrinsic clotting pathway?

#Question id: 10973

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An 11-year-old premenstrual female presents with a painful knee after mild trauma. Upon further evaluation you observe soft tissue bruises. The child is an orphan and there is no family history. The foster mother reports no other problems. The aPTT is prolonged and the PT is normal. A complete hematologic workup would yield

#Question id: 10974

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A 63-year-old woman returned to work following a vacation in New Zealand. Several days after returning home, she awoke with swelling and pain in her right leg and her leg was blue. She went immediately to the emergency room where an examination showed an extensive deep vein thrombosis involving the femoral and iliac veins on the right side. Following resolution of the clot, this patient will require which treatment in the future?

#Question id: 10975

#I Life Science/ Life Sciences Group – I-V

A teenaged boy with numerous nosebleeds was referred to a physician for evaluation prior to a minor surgery. His prothrombin time (PT) was 11 sec (11–15 sec normal), partial thromboplastin time (PTT) was 58 sec (25–40 sec normal), and bleeding time was 6.5 min (2–7 min normal). Which of the following is most likely abnormal in this young man?