Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

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#Question id: 5330

#SCPH06 I Botany

First child of both affected couple has normal, an autosomal dominant disorder. What is probability of second child of this couple would have diseased?

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#Question id: 5241

#SCPH06 I Botany

Genes a, b, and c are widely spaced in the bacterial genome. Transducing phage from an a+ b+ c+ bacterium were used to infect a culture of a- b- c- cells, and b+ transductants were selected. Which of the following best describes the predicted genotypes of these transductants?

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#Question id: 83

#SCPH01 Biochemistry

Which of the following is incorrect about the intramolecular cyclization reaction of glucose in solution ________.

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#Question id: 12747

#SCPH01 Biochemistry

Name the plant protein that bind tightly to specific carbohydrate structure.

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#Question id: 1034

#SCPH28 | Zoology

A new drug is developed that targets and binds to the lipid A portion of LPS from Gram negative bacterial cells. This drug shows a high degree of activity and binding in a test tube setting against purified lipid A. Based on this information,