TLS Online TPP Program

#Question id: 13061


Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Arrange in the order of their increasing capacity:

#SCPH28 | Zoology
  1. Plasmid, BAC, Replacement vectors, Insertion vectors.
  2. Replacement, BAC, Insertion, Plasmid
  3. BAC, Insertion, Replacement, Plasmid
  4. Plasmid, Insertion, Replacement, BAC
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TLS Online TPP Program

#Question id: 4730

#SCPH28 | Zoology

Cross is made between a pure breeding plant having red coloured male flowers with a pure breeding plant having white colored female flowers. All of these offspring are white colored flower. Upon selfing of these F1 red flower plant produced both red and white flower. Which of the following is conclusion of these cross?

TLS Online TPP Program

#Question id: 4731

#SCPH01 Biochemistry

A pure-breeding strain of squash that produced disk-shaped fruits was crossed with a pure breeding strain having long fruits. The F1 had disk fruits, but the F2 showed a new phenotype, sphere, and was composed of the following proportions

Disk  384            

Sphere 96

 Long  32

What is reason for F2 phenotype?

TLS Online TPP Program

#Question id: 4731

#SCPH06 I Botany

A pure-breeding strain of squash that produced disk-shaped fruits was crossed with a pure breeding strain having long fruits. The F1 had disk fruits, but the F2 showed a new phenotype, sphere, and was composed of the following proportions

Disk  384            

Sphere 96

 Long  32

What is reason for F2 phenotype?

TLS Online TPP Program

#Question id: 4731

#SCPH12 I Genetics

A pure-breeding strain of squash that produced disk-shaped fruits was crossed with a pure breeding strain having long fruits. The F1 had disk fruits, but the F2 showed a new phenotype, sphere, and was composed of the following proportions

Disk  384            

Sphere 96

 Long  32

What is reason for F2 phenotype?

TLS Online TPP Program

#Question id: 4731

#SCPH28 | Zoology

A pure-breeding strain of squash that produced disk-shaped fruits was crossed with a pure breeding strain having long fruits. The F1 had disk fruits, but the F2 showed a new phenotype, sphere, and was composed of the following proportions

Disk  384            

Sphere 96

 Long  32

What is reason for F2 phenotype?

TLS Online TPP Program

#Question id: 4732

#SCPH01 Biochemistry

What is F2 phenotype ratio observed when a homozygous recessive mutation in either or both of two different genes results in the same mutant phenotype?