Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Arrange in the order of their increasing capacity:

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#Question id: 55

#SCPH01 Biochemistry

The standard free energy change for the reaction A → B is −15.0 kJ · mol−1. What is the equilibrium constant for the reaction at 25°C?

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#Question id: 18388

#SCPH28 | Zoology

Remediation of Xenobiotics are done because they cause various hazards

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#Question id: 12916

#SCPH01 Biochemistry

Which statement is correct for Zoo blotting?

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#Question id: 406

#I Life Science/ Life Sciences Group – I-V

Hydrolysis of 1 M glucose 6-phosphate catalyzed by glucose 6-phosphatase is 99% complete at equilibrium (i.e., only 1% of the substrate remains). Which of the following statements is most nearly correct? (R = 8.315 J/mol·K; T = 298 K)

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#Question id: 24446

#SCPH06 I Botany

What is the purpose of holter moniter in EKG ?