Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the statement is worng? Aminopetrin………s

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#Question id: 14333

#SCPH05 I Biotechnology

A cylindrical bioreactor of diameter 3 m has four baffles. A Rushton turbine mounted in the reactor has a diameter one third the tank diameter and is operated at a speed of 90 rpm. The density of the fluid is approximately I g cm^-3. The reactor is used to culture an anaerobic organism that does not require gas sparging. The broth can be assumed Newtonian. As the cells grow, the viscosity of the broth increases. when the viscosity is 1000 times greater than water, estimate the power required to achieve turbulence.

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#Question id: 14337

#SCPH05 I Biotechnology

A fermentation broth with viscosity 10 −2 Pa s and density 1000 kg m − 3 is agitated in a 2.7 m^ 3 baffled tank using a Rushton turbine with diameter 0.5 m and stirred speed 1 s ^−1. Estimate the mixing time.

TLS Online TPP Program

#Question id: 14337

#SCPH05 I Biotechnology

A fermentation broth with viscosity 10 −2 Pa s and density 1000 kg m − 3 is agitated in a 2.7 m^ 3 baffled tank using a Rushton turbine with diameter 0.5 m and stirred speed 1 s ^−1. Estimate the mixing time.

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#Question id: 14338

#SCPH05 I Biotechnology

Quasi steady state condition is 

TLS Online TPP Program

#Question id: 14338

#SCPH05 I Biotechnology

Quasi steady state condition is 

TLS Online TPP Program

#Question id: 14339

#SCPH05 I Biotechnology

In continuous culture which phase is usually longer?