Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 12918

#SCPH05 I Biotechnology

Name the enzyme which helps in the DNA synthesis in the PCR.

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#Question id: 15224

#SCPH06 I Botany

Which technique can detect protease activity in sample

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#Question id: 84

#I Life Science/ Life Sciences Group – I-V

Below is the Fischer projection of D-galactose. Which is the proper Haworth projection of β-D-galactopyranose?

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#Question id: 84

#SCPH01 Biochemistry

Below is the Fischer projection of D-galactose. Which is the proper Haworth projection of β-D-galactopyranose?

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#Question id: 15270

#SCPH28 | Zoology

In DNA isolation, for protein free solution what will be the ratio at  nm