To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

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#Question id: 4346

#SCPH01 Biochemistry

Which of the following results would occur if the repressor of an inducible operon were mutated so that it could not bind the operator?

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#Question id: 12925

#SCPH28 | Zoology

Another name of TaqMan assay is ____

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#Question id: 11452

#SCPH28 | Zoology

A given photon of light may trigger an action potential with thousands of times more energy because the signal strength is amplified by ________.

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#Question id: 360

#I Life Science/ Life Sciences Group – I-V

Which of the following is considered to be a strong base (alkali)?

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#Question id: 4171

#SCPH01 Biochemistry

Use the table below to determine which template strand DNA sequence (written in the 5ʹ → 3ʹm direction) specifies the tripeptide with the sequence gly-ala-leu.