9 In your study of a new bacterial species you have identified a transducing phage that you call Px. In addition you have worked out methods to make random transposon insertions into the bacterial genome. You have generated two different transposon insertion collections one with 105 random Tn5 (Kanr) insertions and the other with 105 random Tn10 (Tetr) insertions. You grow Px phage on the mixed collection of Tn5 insertions and use the resulting phage lysate to infect the mixed collection of Tn10 insertions. You select 10,000 Kanr  transductants and find that 80 of them are Tets. Use this information to estimate the total size of the bacterial genome assuming that both Tn5 and Tn10 insert randomly and that the average size of a fragment recombined into the recipient genome during Px transduction is 55 kbp. (Tn5 is about 5 kbp and Tn10 is 10 kbp.)

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#Question id: 2142

#SCPH28 | Zoology

Glucose transport into erythrocytes is an example of

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#Question id: 8937

#SCPH06 I Botany

During metamorphosis, echinoderms undergo a transformation from motile larvae to a sedentary (or sometimes sessile) existence as adults. What differentiates echinoderm adults, but not their larvae? Adults should

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#Question id: 57

#SCPH01 Biochemistry

A(n) ________ has charge but negligible mass, whereas a(n) ________ has mass but no charge.

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#Question id: 6975

#SCPH01 Biochemistry

Which one of the following genes was NOT part of transcription factors used to generate induced pluripotent stem (iPS) cells from mouse skin fibroblasts?

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#Question id: 4152

#SCPH01 Biochemistry

The pathway for polypeptides exported from E. coli includes the following steps, which occur in what order for correct export?

1.  A chaperone, SecA, binds to the polypeptide.

2.  A chaperone, SecB, binds to the polypeptide.

3.  ATP is hydrolyzed by Sec A.

4.  SecA pushes 20 amino acids of the polypeptide into the translocation complex.