What are the molecules mediating signal transduction following antigen binding to cell surface immunoglobulin on a B-cell called?

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#Question id: 37

#SCPH01 Biochemistry

Which one of the following has the cellular components arranged in order of their increasing size?

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#Question id: 10566

#I Life Science/ Life Sciences Group – I-V

What happens if this cell is placed in the beaker containing 0.1 M sucrose, Because the water potential of the sucrose solution (Yw = –0.244) is greater than the water potential of the cell (Yw = –0.732 MPa)

a) Water will move from the cell to the sucrose solution

b) From high to low water potential

c) From low to high water potential

d) Water will move from the sucrose solution to the cell

Which of the following statement is CORRECT?

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#Question id: 13089

#SCPH01 Biochemistry

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;

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#Question id: 18697

#SCPH01 Biochemistry

Knockout mice are created by

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#Question id: 3885

#SCPH06 I Botany

AZT (3'-azido-2',3'-dideoxythymidine), used to treat HIV infection, acts in HIV-infected cells by: