TLS Online TPP Program
More Questions
TLS Online TPP Program
#Question id: 12917
#SCPH28 | Zoology
Arrange the steps of stages in PCR in correct order.
1. The region to be specifically amplified is made accessible within the complex DNA.
2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.
3. A thermal cycler is used to incrementally decrease the annealing temperature.
4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.
TLS Online TPP Program
#Question id: 10511
#SCPH05 I Biotechnology
Which type of pumps inhibits digitoxin in the membranes of heart cells, leading to increased myocardial contraction?
TLS Online TPP Program
#Question id: 18369
#SCPH05 I Biotechnology
The chemical and breakdown products of herbicide DDT cause eggshell thinning and population declines in multiple North American and European bird of prey. They are remediated by
TLS Online TPP Program
#Question id: 3911
#SCPH28 | Zoology
When a sequence of double-strand DNA is displayed
TLS Online TPP Program
#Question id: 13061
#SCPH05 I Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Arrange in the order of their increasing capacity: