The cis conformation shows Cα atoms are on the same side of the peptide bond, is ∼8 kJ mol−1 less stable than the trans conformation. Why?

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#Question id: 16134

#SCPH01 Biochemistry

You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   

 

By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:

 

You determine the DNA sequences of PCR products obtained at STS5 using BACs B, C, D, E, and F as PCR templates. The DNA sequences of PCR products obtained using BACs B and C are identical, but they differ by several nucleotide substitutions from the DNA sequences of PCR products obtained using BACs E and F. Oddly, PCR product obtained using BAC D appears to be a mixture of two sequences – one corresponding to the BAC B/ BAC C sequence and the other corresponding to the BAC E/ BAC F sequence. Briefly account for these findings, and explain how a single PCR assay could amplify two different sequences.

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#Question id: 10779

#I Life Science/ Life Sciences Group – I-V

As plants absorb water from the soil, they deplete the soil of water near the surface of the roots, This depletion due to

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#Question id: 9160

#SCPH06 I Botany

Generally speaking, deserts are located in places where air masses are usually ________.

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#Question id: 15629

#SCPH05 I Biotechnology

Vortexing in a stirred tank reactor can be prevented by using

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#Question id: 13071

#SCPH06 I Botany

Modifications of the agglutination reaction involve the use of ________particles, which allow the reaction to be observed more easily in the liquid phase