TLS Online TPP Program
More Questions
TLS Online TPP Program
#Question id: 16007
#SCPH05 I Biotechnology
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?
TLS Online TPP Program
#Question id: 1301
#SCPH06 I Botany
The cell walls of bacteria, fungi, and plant cells, and the extracellular matrix of animal cells are all external to the plasma membrane. Which of the following characteristics are common to all of these extracellular structures?
TLS Online TPP Program
#Question id: 5553
#I Life Science/ Life Sciences Group – I-V
The notochord is a ___ structure in vertebrate embryos that lies under the ___, and is bordered by ___.
TLS Online TPP Program
#Question id: 19373
#SCPH01 Biochemistry
Third phase, in which all kinds of transgenes are being employed to either modify the quality/quantity of plant/animal produce or to make these organisms produce
TLS Online TPP Program
#Question id: 16134
#SCPH06 I Botany
You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
You determine the DNA sequences of PCR products obtained at STS5 using BACs B, C, D, E, and F as PCR templates. The DNA sequences of PCR products obtained using BACs B and C are identical, but they differ by several nucleotide substitutions from the DNA sequences of PCR products obtained using BACs E and F. Oddly, PCR product obtained using BAC D appears to be a mixture of two sequences – one corresponding to the BAC B/ BAC C sequence and the other corresponding to the BAC E/ BAC F sequence. Briefly account for these findings, and explain how a single PCR assay could amplify two different sequences.