According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

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#Question id: 23248

#SCPH01 Biochemistry

Pyrosequencing is sequencing by synthesis whereby a PCR template is hybridised to an oligonucleotide and incubated with 

1. DNA polymerase

2.  ATP sulphurylase

3. Luciferase 

4. Apyrase

Which of the following option has correct combination?

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#Question id: 5540

#SCPH06 I Botany

A new-born infant attains maternal antibodies from the mother's

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#Question id: 20052

#SCPH01 Biochemistry

HCG-kit is based on the phenomenon of 

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#Question id: 27822

#Research Methodology

Which of the following sampling techniques is commonly used in quantitative research to ensure a representative sample?

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#Question id: 6993

#SCPH28 | Zoology

What molecular marker of head identity is found in the apical tip of a Hydra during head regeneration?