According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

#Question id: 450

#SCPH05 I Biotechnology

The DG'° values for the two reactions shown below are given.

Oxaloacetate + acetyl-CoA + H2O -> citrate + CoASH DG'° = –32.2 kJ/mol

Oxaloacetate + acetate    ->   citrate DG'° = –1.9 kJ/mol

What is the DG'° for the hydrolysis of acetyl-CoA?

Acetyl-CoA + H2O -> acetate + CoASH + H+

#Question id: 450

#SCPH06 I Botany

The DG'° values for the two reactions shown below are given.

Oxaloacetate + acetyl-CoA + H2O -> citrate + CoASH DG'° = –32.2 kJ/mol

Oxaloacetate + acetate    ->   citrate DG'° = –1.9 kJ/mol

What is the DG'° for the hydrolysis of acetyl-CoA?

Acetyl-CoA + H2O -> acetate + CoASH + H+

#Question id: 451

#SCPH05 I Biotechnology

The standard reduction potentials (E'°) for the following half reactions are given.

Fumarate + 2H+ + 2e– -> succinate E'° = +0.031 V

FAD + 2H+ + 2e– -> FADH2 E'° = –0.219 V

If you mixed succinate, fumarate, FAD, and FADH2 together, all at l M concentrations and in the presence of succinate dehydrogenase, which of the following would happen initially?

#Question id: 452

#SCPH05 I Biotechnology

E'° of the NAD+/NADH half reaction is –0.32 V.  The E'° of the oxaloacetate/malate half reaction is –0.175 V.  When the concentrations of NAD+, NADH, oxaloacetate, and malate are all 10–5 M, the “spontaneous” reaction is:

#Question id: 453

#SCPH05 I Biotechnology

Which statement does not explain why many biochemical processes are carried out via multi-step pathways rather than by single-step reactions (or only a few steps)

#Question id: 454

#SCPH05 I Biotechnology

Which statement is false about most metabolic pathways?