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TLS Online TPP Program

#Question id: 13041

Which of the following is incorrect about Data Collection? 

#SCPH01 Biochemistry
  1. The two-color microarray uses multiple dyes at times 
  2. The most common type of microarray protocol is the two-color microarray 
  3. The cDNAs are obtained by extracting total RNA or mRNA from tissues or cells and incorporating fluorescent dyes in the DNA strands during the cDNA biosynthesis 
  4. The expression of genes is measured via the signals from cDNAs hybridizing with the specific oligonucleotide probes on the microarray
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TLS Online TPP Program

#Question id: 14358

#SCPH05 I Biotechnology

One liter per minute of liquid containing A and B (CA0 = 0.10 mollliter, CB() = 0.01 mollliter, Cco=0) flow into a mixed reactor of volume V = 1 liter. The materials react in a complex manner for which the stoichiometry is unknown. The outlet stream from the reactor contains A, B, and C (CAf = 0.02 mollliter, CBf = 0.03 mollliter, Cci = 0.04 mollliter), as shown in fig. 


Find the rate of reaction of A, B, and C for the conditions within the reactor  mol/liter.min 
TLS Online TPP Program

#Question id: 14359

#SCPH05 I Biotechnology

How many enzyme units are there in 1 katal? 
TLS Online TPP Program

#Question id: 14360

#SCPH05 I Biotechnology

1 pg of an enzyme (Mr 160 000) converts 0.192 pmol/min. This enzyme is made up of four subunits. Calculate turnover number 
TLS Online TPP Program

#Question id: 14361

#SCPH05 I Biotechnology

1 m of an enzyme (Mr 160 000) converts 0.192 i.imol/min. This enzyme is made up of four  subunits. Calculate catalytic centre activity
TLS Online TPP Program

#Question id: 14362

#SCPH05 I Biotechnology

An experiment requires the use of 5 x 107 units of enzyme. The price for the enzyme is quoted for katal amounts. Allowing for a 20% excess for wastage, pipetting, and so forth, what approximate round number of katals of enzyme would you purchase? 
TLS Online TPP Program

#Question id: 14363

#SCPH05 I Biotechnology

Enzyme activity was monitored over the last two stages of an enzyme extraction-separation process. 0.4 g protein, from Stage 5, converted 46 mmol substrate in 10 sec, and 0.02 g protein, from Stage 6, converted 49 mmol in 30 sec. Calculate the specific activities in units/g at each of the two steps