Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 3851

#SCPH01 Biochemistry

Transcription is initiated at sites called:

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#Question id: 5351

#SCPH28 | Zoology

Consider the accompanying pedigree of a rare autosomal recessive disease, PKU. (Assume individual coming from outside that is homozygous for normal allele) What is the probability of child showing trait of A and B individuals?

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#Question id: 281

#SCPH12 I Genetics

Histones associate with DNA primarily through ________.

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#Question id: 13109

#SCPH28 | Zoology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 

You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

 

 which number of repeats did Baby C inherit from his mother?

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#Question id: 4567

#I Life Science/ Life Sciences Group – I-V

Charles Darwin was the first person to propose