Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

TLS Online TPP Program

#Question id: 5242

#SCPH06 I Botany

Auxotrohic bacteria plating on different medium, it is observed that colonies grow on minimal plus threonine only, no colony on minimal plus leucine and no colonies grow on minimal medium cystein only. What are the actual genotypes of auxotroph?

TLS Online TPP Program

#Question id: 10878

#I Life Science/ Life Sciences Group – I-V

The resistance of the pulmonary tree is so low that a 1 cm of water pressure gradient is sufficient to cause normal air flow during resting conditions. Which of the following often has a substantial resistance during pulmonary disease states that can limit alveolar ventilation?

TLS Online TPP Program

#Question id: 359

#SCPH05 I Biotechnology

What is the concentration of OH− in a solution with an H+ concentration of 1.3 × 10^−4 M?

TLS Online TPP Program

#Question id: 16130

#SCPH01 Biochemistry

You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS.              

How many units of ß-galactiosidase would you expect to be expressed from deletion 1 in a gln2– mutant? 

TLS Online TPP Program

#Question id: 18647

#SCPH01 Biochemistry

Which of the following technique used discontinuous type gel system