TLS Online TPP Program

#Question id: 13056


Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
 Which of the following is true for traditional and real time PCR?

#SCPH01 Biochemistry
  1. Both the techniques allow detection of the product in early stages
  2. Real time PCR can detect the product in early stages as compared to traditional PCR
  3. Both systems require different primers
  4. Both techniques are end point product determination methods

More Questions
TLS Online TPP Program

#Question id: 18385

#SCPH28 | Zoology

Oil is a natural product, has many components and is biodegradable, the different components being degraded at different rates but still oil spills are considered as recalciterants because

TLS Online TPP Program

#Question id: 18386

#SCPH01 Biochemistry

Biomagnification occurs mainly because of the following reasons
I. compounds are taken up from the environment and accumulated in the lipid deposits of body
II. organisms are consumed by other organisms in a non- sequential manner constituting the food chain
Which of the above statements are incorrect?

TLS Online TPP Program

#Question id: 18386

#SCPH05 I Biotechnology

Biomagnification occurs mainly because of the following reasons
I. compounds are taken up from the environment and accumulated in the lipid deposits of body
II. organisms are consumed by other organisms in a non- sequential manner constituting the food chain
Which of the above statements are incorrect?

TLS Online TPP Program

#Question id: 18386

#SCPH06 I Botany

Biomagnification occurs mainly because of the following reasons
I. compounds are taken up from the environment and accumulated in the lipid deposits of body
II. organisms are consumed by other organisms in a non- sequential manner constituting the food chain
Which of the above statements are incorrect?

TLS Online TPP Program

#Question id: 18386

#SCPH28 | Zoology

Biomagnification occurs mainly because of the following reasons
I. compounds are taken up from the environment and accumulated in the lipid deposits of body
II. organisms are consumed by other organisms in a non- sequential manner constituting the food chain
Which of the above statements are incorrect?

TLS Online TPP Program

#Question id: 18387

#SCPH01 Biochemistry

Structural features that make these compounds resistant to microbial degradation include
1. substitution of H by other groups
2. heterocyclic compounds
3. branched linear chains
Which of the above are correct?