Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

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#Question id: 3895

#SCPH28 | Zoology

Which one of the following types of eukaryotic regulatory proteins interact with enhancers?

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#Question id: 18903

#SCPH01 Biochemistry

For imaging purpose, a specialized gamma camera is used to detect the radiation throughout the body. Most used techniques in nuclear imaging are 

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#Question id: 15618

#SCPH28 | Zoology

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1-, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac-). During an condition  isolate  a second Lac– mutation, which you designate lac2-. Using P1 phage on this strain and use the resulting phage lysate to infect the lac2- strain, selecting for   Kanr   transductants. In this case, all 100   Kanr   transductants that are examined are Lac–. What does this result tell you about the relationship between the lac1- and lac2- mutations?

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#Question id: 3869

#SCPH05 I Biotechnology

Which one of the following statements about E. coli RNA polymerase (core enzyme) is false?

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#Question id: 12572

#SCPH06 I Botany

Use what you know about ecosystem stability and the information provided in the graph below to answer the following question.

Which community (a-e) would likely be the most biodiverse?