Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 3642

#SCPH06 I Botany

Any piece of DNA which replicates as a single unit is called

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#Question id: 9196

#SCPH06 I Botany

If all individuals in the last remaining population of a particular frog species were all highly elated, which type of diversity would be of greatest concern when planning to prevent the species from going extinct?

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#Question id: 8921

#SCPH05 I Biotechnology

Evidence of which structure or characteristic would be most surprising to find among fossils of the Ediacaran fauna?

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#Question id: 11598

#SCPH06 I Botany

How the GA3 is differ from GA1______

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#Question id: 11026

#I Life Science/ Life Sciences Group – I-V

Listed below are the hydrostatic and oncotic pressures and filtration rate across a muscle capillary wall:

Capillary hydrostatic pressure (Pc) = 25 mm Hg

Plasma colloid osmotic pressure (Πp) = 25 mm Hg

Interstitial colloid osmotic pressure (ΠI) = 10 mm Hg

Interstitial hydrostatic pressure (PI) = −5 mm Hg

Capillary filtration rate = 150 ml/min

What is the capillary filtration coefficient?