Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

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#Question id: 18624

#SCPH01 Biochemistry

In GFP protein oxidation of tripeptide sequence gives fluorescence the tripeptide sequence made up of

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#Question id: 23170

#SCPH06 I Botany

Which epitope tags can be used to tag recombinant proteins?

1. FLAG

2. myc

3. TICK

Which of the following options is incorrect?

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#Question id: 345

#SCPH28 | Zoology

As the [H₃O⁺] of the solution decreases, the [OH-] ________.

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#Question id: 10527

#SCPH06 I Botany

Based on their feeding behaviors, three major types of insect herbivores can be distinguished, which evades the constitutive defense;

A) Phloem feeders                                                                    i) caterpillars, grasshoppers, and beetles

B) Cell-content feeders                                                            ii) aphids and whiteflies

C) Chewing insects                                                                    iii) mites and thrips

Correctly match the feeding behaviour with its insects;

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#Question id: 5323

#SCPH28 | Zoology

An X-linked recessive gene produces red-green color blindness in humans. A woman with normal color vision whose father was color-blind marries a color-blind man. What is the probability that their son will be color-blind?