Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

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#Question id: 14737

#SCPH05 I Biotechnology

Which structures are involved in formation of biofilms

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#Question id: 14737

#SCPH06 I Botany

Which structures are involved in formation of biofilms

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#Question id: 14741

#SCPH05 I Biotechnology

Microbial cell recovery is carried out in a continuous disc-stack centrifuge. The centrifuge is operated at 5000 rpm for separation of baker's yeast. At a feed rate of 60 1/min, 50% of the cells are recovered. At constant speed, solid recovery is inversely proportional to flow rate. What operating speed is required to recover 90% of cells if the flow rate in the centrifuge is maintained at 601/min? 

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#Question id: 14742

#SCPH05 I Biotechnology

Gel chromatography is used for hormone separation. A pilot-plant scale gel chromatography column packed with sephacryl resin is used to separate two hormones, A and B. The column is 5 cm in diameter and 30 cm in height: V, = 1.9x 10-⁴m³, Wr = 3x10^-3m³ /kg dry sephacryl, ps =1.25 x 10³ kg/m³ . The partition coefficients are k_pA =0.38 and k_pB= 0.15. If the eluting flow rate is 0.7 I/h, what is the retention time for each hormone? 

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#Question id: 14743

#SCPH05 I Biotechnology

for the fermenter with the given data. 

Air is sparged with 0.4 vvm, it is equipped with two sets of impellers and a flat-blade turbine with four baffles. calculate power, P 

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#Question id: 14744

#SCPH05 I Biotechnology

For production of an enzyme used for synthesis of a sun protection lotion, the required kinetic data and constants are: 

vmax = 2.3 mmol.m^-3 s^-1 Km = 8.9 mmol.^-1 So = 12 mmol1- 

Find the reaction time for 95% conversion of raw material