TLS Online TPP Program

#Question id: 13058


Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following true about real time PCR?

#SCPH01 Biochemistry
  1. It is an end point quantification method
  2. It is a method for real time quantification of the PCR products
  3. It is fluorescent probe free method of quantification
  4. After real time PCR product can only be detected at the end of the PCR
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TLS Online TPP Program

#Question id: 11272

#SCPH06 I Botany

Measurement of phytoplankton production, take sample of phytoplankton suspended in three bottles in lake. One bottle as control assumed as initial, second bottle (D) was kept in dark while third bottle (L) was kept in light. After some time productivity measure fallow

A- Difference in dissolved oxygen between light bottle and dark bottle yield NET primary productivity

B- Difference in dissolved oxygen between light bottle and initial bottle yield NET primary productivity

C- Difference in dissolved oxygen between light bottle and initial bottle yield Grass primary productivity

D- Difference in dissolved oxygen between light bottle and dark bottle yield total oxygen produced by photosynthesis

Which above combination of statement are correct?

TLS Online TPP Program

#Question id: 11272

#SCPH28 | Zoology

Measurement of phytoplankton production, take sample of phytoplankton suspended in three bottles in lake. One bottle as control assumed as initial, second bottle (D) was kept in dark while third bottle (L) was kept in light. After some time productivity measure fallow

A- Difference in dissolved oxygen between light bottle and dark bottle yield NET primary productivity

B- Difference in dissolved oxygen between light bottle and initial bottle yield NET primary productivity

C- Difference in dissolved oxygen between light bottle and initial bottle yield Grass primary productivity

D- Difference in dissolved oxygen between light bottle and dark bottle yield total oxygen produced by photosynthesis

Which above combination of statement are correct?

TLS Online TPP Program

#Question id: 11273

#SCPH06 I Botany

Use the following diagram of a hypothetical food web to answer the question. The arrows represent the transfer of energy between the various trophic levels. Which letter represents an organism that could only be a primary consumer and secondary carnivores respectively?




TLS Online TPP Program

#Question id: 11273

#SCPH28 | Zoology

Use the following diagram of a hypothetical food web to answer the question. The arrows represent the transfer of energy between the various trophic levels. Which letter represents an organism that could only be a primary consumer and secondary carnivores respectively?




TLS Online TPP Program

#Question id: 11274

#SCPH06 I Botany

Assimilation efficiency (AE) is the proportion of ingested energy assimilated in to blood stream but Production efficiency (PE) is the proportion of assimilated energy that is incorporated in to biomass. Following some statement is correct?

A. Assimilation efficiencies of aquatic herbivore is greater than terrestrial herbivore

B. Production efficiencies of higher tropic level are greater than lower tropic level

C. production efficiencies of vertebrate is less than invertebrate

D. Assimilation efficiencies of Carnivore are often greater than herbivore

TLS Online TPP Program

#Question id: 11274

#SCPH28 | Zoology

Assimilation efficiency (AE) is the proportion of ingested energy assimilated in to blood stream but Production efficiency (PE) is the proportion of assimilated energy that is incorporated in to biomass. Following some statement is correct?

A. Assimilation efficiencies of aquatic herbivore is greater than terrestrial herbivore

B. Production efficiencies of higher tropic level are greater than lower tropic level

C. production efficiencies of vertebrate is less than invertebrate

D. Assimilation efficiencies of Carnivore are often greater than herbivore