Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

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#Question id: 19469

#SCPH01 Biochemistry

Glyphosate is extensively and rapidly metabolised by glyphosate oxidoreductase (gox), which breaksdown glyphosate into 

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#Question id: 14252

#SCPH05 I Biotechnology

Yeast cells are recovered from a fermentation broth by using a tubular centrifuge. Sixty percent (60%) of the cells are recovered at a flow rate of 12 l/min with a rotational speed of 4000 rpm. Recovery is inversely proportional to flow rate. At a constant rpm of 4000 rpm, what should be the flow rate to result in 95% cell recovery?

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#Question id: 10246

#SCPH06 I Botany

Under an ideal free distribution:

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#Question id: 6969

#I Life Science/ Life Sciences Group – I-V

In Xenopus, the elongation of the mesoderm toward the anterior results from the intercalation of cells during a process called:

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#Question id: 15273

#SCPH01 Biochemistry

In RNA isolation, for protein free solution what will be the ratio at  nm