Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

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#Question id: 2122

#SCPH06 I Botany

The inner (plasma) membrane of E. coli is about 75% lipid and 25% protein by weight.  How many molecules of membrane lipid are there for each molecule of protein?  (Assume that the average protein is Mr 50,000 and the average lipid is 750.)

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#Question id: 2668

#SCPH05 I Biotechnology

The toxic shock syndrome toxin is produced by some Salmonella species when there is not sufficient iron present. When iron is present, it prevents transcription of the TSS-toxin gene. Iron therefore serves as __________.

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#Question id: 19708

#SCPH06 I Botany

In complete heart block

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#Question id: 5690

#SCPH28 | Zoology

Species I is diploid (2n =4) with chromosomes AABB; related species II is diploid (2n = 6) with chromosomes MMNNOO .Which of the following chromosome  in allotriploid individuals?

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#Question id: 12902

#SCPH01 Biochemistry

An extension of microarray technology may also be used to analyze tissue sections,this process is known as_____