Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 15212

#SCPH28 | Zoology

Sieving effect that contributes to the separation of proteins according to their size, in techniques such as SDS–gel electrophoresis, the concentration of gel used

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#Question id: 17080

#SCPH28 | Zoology

Hypoxanthine and thymidine are required for running which pathway

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#Question id: 22940

#SCPH28 | Zoology

In rice, a SDP, homolog of CONSTANS gene is 

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#Question id: 9271

#I Life Science/ Life Sciences Group – I-V

Chlorophylls a and b are abundant in green plants, and c and d are found in some protists and cyanobacteria. A number of different types of bacteriochlorophyll have been found; which type of chlorophyll is the most widely distributed, shows the distribution of pigments in different types of photosynthetic organisms;

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#Question id: 6972

#SCPH01 Biochemistry

Sodium ions influx and passive water movement result in fluid-filled blastocoel in __________embryos.