Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Agglutination of antigens by utilising specific antibodies can only occur, if

#Question id: 368

#SCPH05 I Biotechnology

Which of the following is true about the properties of aqueous solutions?

#Question id: 40

#SCPH06 I Botany

Given that the standard free-energy change for the reaction glucose + Pi → glucose 6-phosphate is 13.8 kJ/mol, and the standard free-energy change for the reaction ATP → ADP + Pi is −30.5 kJ/mol, what is the free-energy change for the reaction glucose + ATP → glucose 6-phosphate + ADP?

#Question id: 4159

#SCPH01 Biochemistry

Which statement is not true about the ʺwobbleʺ position?

#Question id: 5443

#SCPH28 | Zoology

Two varieties of maize averaging 25 and 125 inches in height, respectively, are crossed. The F1 progeny is quite uniform averaging 75 inches in height. Plant height is control by 3 QTL, what is the probability of plant with 25 inch height in F2 generation?

#Question id: 10507

#SCPH05 I Biotechnology

Rhizobacteria function as

 a) Nitrogen fixation

 b) depositing pathogens directly into the vascular system,

 c) producing antibiotics and antifungal agents for inhibiting soil pathogens

 d) herbivore sensors by sending electrical or chemical signals to surrounding cells

 Which one of the following combination is correct?