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#Question id: 13066


Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following is a codominant marker?

#SCPH01 Biochemistry
  1. RFLP and AFLP
  2. RFLP and RAPD
  3. AFLP only
  4. RAPD only
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TLS Online TPP Program

#Question id: 11606

#SCPH06 I Botany

What will be the effect light in GA biosynthesis?

a) Light basically activates the GA 3β-hydroxylase which adds a hydroxyl group (OH) to carbon 3 of GA20 and convert GA20 to GA1

b) light-induced increase in the transcription of the gene for GA3ox, which converts GA20 to GA1

c) light-induced increase in the transcription of the gene for GA2ox, which converts GA20 to GA29

d) light-induced decreases the transcription of the gene for GA3ox, which converts GA20 to GA1

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#Question id: 8682

#SCPH06 I Botany

Giardia intestinalis can cause disease in several different mammalian species, including humans. Giardia organisms (G. intestinalis) that infect humans are similar morphologically to those that infect other mammals, thus they have been considered a single species. However, G. intestinalis has been divided into different subgroups based on their host and a few other characteristics. In 1999, a DNA sequence comparison study tested the hypothesis that these subgroups actually constitute different species. The following phylogenetic tree was constructed from the sequence comparison of rRNA from several subgroups of G. intestinalis and a few other morphologically distinct species of Giardia. The researchers concluded that the subgroups of Giardia are sufficiently different from one another genetically that they could be considered different species


Which of the following changes would a modern systematist be most likely to make after learning of the results of the rRNA analyses?

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#Question id: 18390

#SCPH06 I Botany

Which bacterium is mainly found at quite high ionizing radiation zones or areas?

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#Question id: 14281

#SCPH05 I Biotechnology

cells are grown in a CSTR a steady state at two dilution rates 0.1 h^-1 and 0.5h^-1 and the steady state concentrations are as follows: 


if the inlet substrate concentration is 20 g/I, the cells follow monod growth kinetics and product formation kinetics is modelled as a both growth associated and non growth associated kinetics given by the equation then answer the following questions, the best estimate of maximum specific growth rate is: 

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#Question id: 6977

#SCPH28 | Zoology

Cartilage (bone-forming tissue) and connective tissue of the vertebrate limb form from: