To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

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#Question id: 15471

#SCPH05 I Biotechnology

Which one of the following need NOT be conserved in a biochemical reaction?

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#Question id: 2325

#SCPH28 | Zoology

Which of the following is found in BOTH prokaryotic and eukaryotic cells?

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#Question id: 8902

#SCPH28 | Zoology

What do craniates have that earlier chordates did not have?

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#Question id: 5111

#SCPH01 Biochemistry

Once the germ layers are established, the cells interact with one another and rearrange themselves to produce tissues and organs. This process is called:

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#Question id: 1037

#SCPH28 | Zoology

In a rapidly multiplying bacterial population, cell numbers increase