To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

#Question id: 4918

#SCPH28 | Zoology

The two organelles responsible for cytoplasmic inheritance among eukaryotes are

#Question id: 4919

#SCPH06 I Botany

Genes for cytoplasmic male sterility in plants are generally located in

#Question id: 4919

#SCPH12 I Genetics

Genes for cytoplasmic male sterility in plants are generally located in

#Question id: 4919

#SCPH28 | Zoology

Genes for cytoplasmic male sterility in plants are generally located in

#Question id: 4920

#SCPH06 I Botany

In Neurospora, the mutant stp exhibits erratic stop-and start growth. When a female of stp strain is crossed with a normal strain acting as a male, all progeny individuals' showed stp mutant phenotype. However, the reciprocal cross resulted in all normal progeny, individuals. These results can be explained on the basis of

A. Uniparental gene contribution

B. Nuclear inheritance with concept of dominance

C. Sex link character

D. stp mutation may be located in chloroplast DNA

The most appropriate statement or combination of the above statements for explaining the experimental results is:

#Question id: 4920

#SCPH12 I Genetics

In Neurospora, the mutant stp exhibits erratic stop-and start growth. When a female of stp strain is crossed with a normal strain acting as a male, all progeny individuals' showed stp mutant phenotype. However, the reciprocal cross resulted in all normal progeny, individuals. These results can be explained on the basis of

A. Uniparental gene contribution

B. Nuclear inheritance with concept of dominance

C. Sex link character

D. stp mutation may be located in chloroplast DNA

The most appropriate statement or combination of the above statements for explaining the experimental results is: