To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;

#Question id: 10527

#I Life Science/ Life Sciences Group – I-V

Based on their feeding behaviors, three major types of insect herbivores can be distinguished, which evades the constitutive defense;

A) Phloem feeders                                                                    i) caterpillars, grasshoppers, and beetles

B) Cell-content feeders                                                            ii) aphids and whiteflies

C) Chewing insects                                                                    iii) mites and thrips

Correctly match the feeding behaviour with its insects;

#Question id: 10527

#SCPH05 I Biotechnology

Based on their feeding behaviors, three major types of insect herbivores can be distinguished, which evades the constitutive defense;

A) Phloem feeders                                                                    i) caterpillars, grasshoppers, and beetles

B) Cell-content feeders                                                            ii) aphids and whiteflies

C) Chewing insects                                                                    iii) mites and thrips

Correctly match the feeding behaviour with its insects;

#Question id: 10527

#SCPH05 I Biotechnology

Based on their feeding behaviors, three major types of insect herbivores can be distinguished, which evades the constitutive defense;

A) Phloem feeders                                                                    i) caterpillars, grasshoppers, and beetles

B) Cell-content feeders                                                            ii) aphids and whiteflies

C) Chewing insects                                                                    iii) mites and thrips

Correctly match the feeding behaviour with its insects;

#Question id: 10527

#SCPH06 I Botany

Based on their feeding behaviors, three major types of insect herbivores can be distinguished, which evades the constitutive defense;

A) Phloem feeders                                                                    i) caterpillars, grasshoppers, and beetles

B) Cell-content feeders                                                            ii) aphids and whiteflies

C) Chewing insects                                                                    iii) mites and thrips

Correctly match the feeding behaviour with its insects;

#Question id: 10528

#I Life Science/ Life Sciences Group – I-V

The insect ingests plant tissue containing the fatty acids, an enzyme in the gut conjugates the plant-derived fatty acid to an insect-derived amino acid typically;

#Question id: 10528

#SCPH05 I Biotechnology

The insect ingests plant tissue containing the fatty acids, an enzyme in the gut conjugates the plant-derived fatty acid to an insect-derived amino acid typically;