To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 1421

#SCPH28 | Zoology

XIAP proteins:

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#Question id: 10990

#SCPH28 | Zoology

Sympathetic stimulation of the heart normally causes which of the following conditions?

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#Question id: 3861

#SCPH28 | Zoology

_ are sequences that are removed from primary transcripts, and _ are sequences that remain present in mature mRNA molecules.

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#Question id: 14278

#SCPH05 I Biotechnology

Stirrer has a power number (Np) 10. If the stirrer speed (N) is 1s^-1, its diameter D=1m and the density of the medium is 1000 kg/m^3, the ungassed power input (P) in watts is 

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#Question id: 6954

#SCPH28 | Zoology

Between fertilization and the first cleavage division, actin filaments pull the cytoplasm of the frog egg toward the point of sperm entry in a process called: