TLS Online TPP Program

#Question id: 13104


With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.
 

 
Why is it that some people show two bands?

#SCPH01 Biochemistry
  1. They are heterozygotes at this region.
  2. They are homozygotes at this region.
  3. Because longer the DNA fragments.
  4. Because the more repeats is found.

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TLS Online TPP Program

#Question id: 23147

#SCPH06 I Botany

In fluorescence microscopy why the fluorescence occurs at longer wavelength when there shorter   wavelength of light is applied?

TLS Online TPP Program

#Question id: 23148

#SCPH06 I Botany

There are two proteins A and B to be visualized under fluorescence microscopy. Both of them are excited with same wavelength of light but A emits longer wavelength. Hence we can conclude that 
1. A is denser molecule than B
2. B is denser molecule than A
3. A is lighter molecule than B
4. B is lighter molecule than A
Which of the following option is incorrect?

TLS Online TPP Program

#Question id: 23149

#SCPH06 I Botany

In fluorescence microscopy a usually used fluorochrome that is fura-2 is sensitive to which ions?

TLS Online TPP Program

#Question id: 23150

#SCPH06 I Botany

How fura-2 dye moves inside the cell through plasma membrane?

TLS Online TPP Program

#Question id: 23151

#SCPH06 I Botany

If the concentration of Ca2+ is high inside the cell and fura-2 dye is used to map the concentration which of the following conditions will prevail?
1. Fluorescence will decrease
2.  Fluorescence will increase
3. Emission of light at higher wavelength
4. Emission of light at lower wavelength
Which of the following options are correct?

TLS Online TPP Program

#Question id: 23152

#SCPH06 I Botany

A fluorescent dye SNARF-1 that is sensitive to H+ concentrations can be used to monitor the cytosolic pH of 
1. Alkaline cells
2. Acidic cells 
3. Dead cells
4. Live cells
Which of the following options are incorrect?