#Question id: 14163
#SCPH05 I Biotechnology
A stream of growth medium containing 60% glucose and 40% growth factor A flowing at 500 kg per hour was divided into two feed streams for different cell cultures. One fourth of the growth medium was sent to fermenter 1 and the rest was sent to fermenter 2. What is the flow rates of glucose and growth factor A in the feed streams for the two fermenters
#Question id: 14164
#SCPH05 I Biotechnology
Stoichiometric equations are used to represent growth of
microorganisms provided a ' molecular formula' for the cells is available. The
molecular formula for biomass is obtained by measuring the amounts of C, N, H,
O and other elements in cells. For a particular bacterial strain, the molecular
formula was determined to be C4.4H7.30 1.2N0.86
. These bacteria are grown under aerobic conditions with hexadecane (C16H34)
as substrate. The reaction equation describing growth is:
Assuming 100% conversion, what is the yield of cells from hexadecane in g g-l?
#Question id: 14165
#SCPH05 I Biotechnology
Stoichiometric equations are used to represent growth of
microorganisms provided a ' molecular formula' for the cells is available. The
molecular formula for biomass is obtained by measuring the amounts of C, N, H,
O and other elements in cells. For a particular bacterial strain, the molecular
formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown
under aerobic conditions with hexadecane (C16H34) as substrate. The reaction
equation describing growth is:
Assuming 100% conversion, what is the yield of cells from oxygen in g g-l?
#Question id: 14166
#SCPH05 I Biotechnology
Stoichiometric equations are used to represent growth of
microorganisms provided a ' molecular formula' for the cells is available. The
molecular formula for biomass is obtained by measuring the amounts of C, N, H,
O and other elements in cells. For a particular bacterial strain, the molecular
formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown
under aerobic conditions with hexadecane (C16H34) as substrate. The reaction
equation describing growth is:
You have been put in charge of a small fermenter for growing the bacteria and aim to produce 2.5 kg of cells for inoculation of a pilot-scale reactor. What minimum amount of hexadecane substrate must be contained in your culture medium?
#Question id: 14167
#SCPH05 I Biotechnology
Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest can be considered solids. Beet molasses containing 50% sucrose, 1% invert sugars, 18% water and the remainder solids, is mixed with corn-steep liquor in a mixing tank. Water is added to produce a diluted sugar mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor and 45 kg molasses are fed into the tank.
How much water is required?
#Question id: 14168
#SCPH05 I Biotechnology
Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest can be considered solids. Beet molasses containing 50% sucrose, 1% invert sugars, 18% water and the remainder solids, is mixed with corn-steep liquor in a mixing tank. Water is added to produce a diluted sugar mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor and 45 kg molasses are fed into the tank.
What is the concentration of solids in the final mixture?