Myeloma is a cancer of the plasma cells, human a patient with myeloma, these patient produces 

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#Question id: 14158

#SCPH01 Biochemistry

BLAST uses a _______ to find matching words, whereas FASTA identifies identical matching words using the _____

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#Question id: 15623

#SCPH05 I Biotechnology

Under unaerated condition in a fermentor, the power consumed by a single impeller was 10,000 W. The diameter of the impeller D is 1m and the density of the medium is 1000 kg/m3 and the stirrer speed is 1s^-1. If one more impeller is added to the fermentor, power consumption would be approximately

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#Question id: 353

#SCPH05 I Biotechnology

Measurements show that the pH of a particular lake is 4.0. What is the hydrogen ion concentration of the lake?

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#Question id: 11544

#SCPH28 | Zoology

When a steroid hormone and a polypeptide hormone exert similar effects on a population of target cells, then ________.

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#Question id: 13088

#SCPH28 | Zoology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;