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#Question id: 15652
#SCPH01 Biochemistry
You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium. You have isolated a mutation that causes constitutive melibiase activity, which you designate MelA–. P1 phage mapping experiments using a Tn5 insertion linked to Mel1 show that MelA– is not linked to Mel1. Moreover you find that when an amber suppressor is introduced into a MelA– mutant, normal melibiase regulation is restored. Classify the MelA– mutation in terms of its basic genetic properties,
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#Question id: 2261
#SCPH05 I Biotechnology
Oxidative metabolism is carried out ____ of mitochondria.
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#Question id: 23247
#SCPH06 I Botany
In pyrosequencing which molecule is actually detected?
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#Question id: 15226
#SCPH06 I Botany
DNA from Salmonella species, when digested with the restriction enzyme Xba I, gives around 15 fragments ranging from 25 kb to 680 kb This pattern of fragments, or ‘fingerprint’, is unique to that strain. If the same fingerprint is found from bacteria from other infected people, then it can be assumed that they were all infected from a common source. Thus, by comparing their eating habits, food sources, etc. the source of the infection can be traced to a restaurant, food item. The fingerprints are created and analysed by
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#Question id: 668
#SCPH05 I Biotechnology
What feature does a Ramachandran plot display?