You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium.

Next, you isolate a third mutant, MelC–, which gives constitutive melibiase expression. The MelC– mutation is closely linked to Mel1 and MelB–. Genetic tests of the MelC– mutation yield the following:

 

As above, classify the MelC– mutation in terms of its basic genetic properties and explain how you arrived at your conclusions.

TLS Online TPP Program

#Question id: 18614

#SCPH01 Biochemistry

The primer length affects the efficiency rate of PCR, this rate decreases as the primer length

TLS Online TPP Program

#Question id: 18614

#SCPH06 I Botany

The primer length affects the efficiency rate of PCR, this rate decreases as the primer length

TLS Online TPP Program

#Question id: 18615

#SCPH01 Biochemistry

PCR efficiency is influenced by several factors, the efficiency declines due to

a) efficiency of amplification declines with an increase in the length of the target sequence.

b) Efficiency of PCR is affected by primer length; it declines if the primers used for PCR arc too long.

c) a temperature higher than the ideal annealing temperature reduces PCR efficiency.

d)  primers used for PCR have complementary regions, dimers will be formed, and PCR efficiency will decline.

TLS Online TPP Program

#Question id: 18615

#SCPH06 I Botany

PCR efficiency is influenced by several factors, the efficiency declines due to

a) efficiency of amplification declines with an increase in the length of the target sequence.

b) Efficiency of PCR is affected by primer length; it declines if the primers used for PCR arc too long.

c) a temperature higher than the ideal annealing temperature reduces PCR efficiency.

d)  primers used for PCR have complementary regions, dimers will be formed, and PCR efficiency will decline.

TLS Online TPP Program

#Question id: 18616

#SCPH01 Biochemistry

In a PCR higher annealing temperature along with7-dcaza guanosine triphosphate (7-deaza OTP) used in place of GTP this indicate

TLS Online TPP Program

#Question id: 18616

#SCPH06 I Botany

In a PCR higher annealing temperature along with7-dcaza guanosine triphosphate (7-deaza OTP) used in place of GTP this indicate