TLS Online TPP Program

#Question id: 16129


5 You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS.
Describe the cis-acting elements in the GLN1 promoter that are evident from these experiments, giving both their position and as much of their function as you can deduce.

#SCPH01 Biochemistry
  1. Deletion1 : GLN2 function, Deletions 3, 4 : GLN3 function, contain TATA, Deletion 6: sequence contain UAS Sequence -involved in activation.
  2. Deletion1 : GLN3 function, Deletions 3, 4 : GLN2 function, contain UAS Sequence -involved in activation, Deletion 6: contain TATA sequence.
  3. Deletion1 : GLN2 function, Deletions 3, 4 : GLN3 function, contain UAS Sequence -involved in activation, Deletion 6: contain TATA sequence.
  4. Deletion1, 3 and 4 : GLN3 function, contain UAS Sequence -involved in activation, Deletion 6: contain TATA sequence.
More Questions
TLS Online TPP Program

#Question id: 5204

#SCPH28 | Zoology

Bioinformatics can be used to scan sequences for probable genes looking for start and stop sites for transcription and for translation, for probable splice sites, and for sequences known to be found in other known genes. Such sequences containing these elements are called

TLS Online TPP Program

#Question id: 5205

#SCPH06 I Botany

Why is it preferable to use large computers and databases in searching for individual genes, rather than testing each sequence for possible function?

TLS Online TPP Program

#Question id: 5205

#SCPH28 | Zoology

Why is it preferable to use large computers and databases in searching for individual genes, rather than testing each sequence for possible function?

TLS Online TPP Program

#Question id: 5206

#SCPH06 I Botany

A microarray known as a GeneChip, with most now known human protein coding sequences, has recently been developed to aid in the study of human cancer by first comparing twothree subsets of cancer subtypes. What kind of information might be gleaned from this GeneChip to aid in cancer prevention?

TLS Online TPP Program

#Question id: 5206

#SCPH28 | Zoology

A microarray known as a GeneChip, with most now known human protein coding sequences, has recently been developed to aid in the study of human cancer by first comparing twothree subsets of cancer subtypes. What kind of information might be gleaned from this GeneChip to aid in cancer prevention?

TLS Online TPP Program

#Question id: 5207

#SCPH06 I Botany

Why is it unwise to try to relate an organismʹs complexity with its size or number of cells?