Practice Questions

TLS Online TPP Program

#Question id: 16135

You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.

By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:

Would you expect the DNA sequence of PCR product obtained at STS5 using mouse genomic DNA as template to more closely resemble that obtained using BAC B, BAC D, or BAC F? Briefly explain your answer.

#SCPH01 Biochemistry

STS 5 in twice our BACS, there are probably at least one STS 5 on the mouse chromosome. Only BAC B contains STS5s.
STS 5 in twice our BACS, there are probably at least two STS 5 on the mouse chromosome. Only BAC D contains both STS5s.
STS 5 present in only one our BACS, there are probably at least one STS 5 on the mouse chromosome. Only BAC F contains STS5s.
STS 5 in twice our BACS, there are probably at least two STS 5 on the mouse chromosome. BAC B and F contains both STS5s.