TLS Online TPP Program

#Question id: 16135


You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   
 
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
 
Would you expect the DNA sequence of PCR product obtained at STS5 using mouse genomic DNA as template to more closely resemble that obtained using BAC B, BAC D, or BAC F? Briefly explain your answer.

#SCPH01 Biochemistry
  1. STS 5 in twice our BACS, there are probably at least one STS 5 on the mouse chromosome. Only BAC B contains  STS5s.
  2. STS 5 in twice our BACS, there are probably at least two STS 5 on the mouse chromosome. Only BAC D contains both STS5s.
  3. STS 5 present in only one our BACS, there are probably at least one STS 5 on the mouse chromosome. Only BAC F contains  STS5s.
  4. STS 5 in twice our BACS, there are probably at least two STS 5 on the mouse chromosome. BAC B and F contains both STS5s.
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TLS Online TPP Program

#Question id: 562

#SCPH06 I Botany

Phenyl-methane-sulfonyl-fluoride (PMSF) inactivates serine proteases by binding covalently to the catalytic serine residue at the active site; this enzyme-inhibitor bond is not cleaved by the enzyme.  This is an example of what kind of inhibition?

TLS Online TPP Program

#Question id: 562

#SCPH28 | Zoology

Phenyl-methane-sulfonyl-fluoride (PMSF) inactivates serine proteases by binding covalently to the catalytic serine residue at the active site; this enzyme-inhibitor bond is not cleaved by the enzyme.  This is an example of what kind of inhibition?

TLS Online TPP Program

#Question id: 563

#SCPH01 Biochemistry

Both water and glucose share an —OH that can serve as a substrate for a reaction with the terminal phosphate of ATP catalyzed by hexokinase.  Glucose, however, is about a million times more reactive as a substrate than water.  The best explanation is that:

TLS Online TPP Program

#Question id: 563

#I Life Science/ Life Sciences Group – I-V

Both water and glucose share an —OH that can serve as a substrate for a reaction with the terminal phosphate of ATP catalyzed by hexokinase.  Glucose, however, is about a million times more reactive as a substrate than water.  The best explanation is that:

TLS Online TPP Program

#Question id: 563

#SCPH06 I Botany

Both water and glucose share an —OH that can serve as a substrate for a reaction with the terminal phosphate of ATP catalyzed by hexokinase.  Glucose, however, is about a million times more reactive as a substrate than water.  The best explanation is that:

TLS Online TPP Program

#Question id: 563

#SCPH28 | Zoology

Both water and glucose share an —OH that can serve as a substrate for a reaction with the terminal phosphate of ATP catalyzed by hexokinase.  Glucose, however, is about a million times more reactive as a substrate than water.  The best explanation is that: