qPCR can be done by 

i) cyanin dye or SYBR green – to measure amplification

ii) Taqman assay – measure extension

iii) molecular beacons - denaturation

TLS Online TPP Program

#Question id: 18074

#SCPH05 I Biotechnology

Affinity chromatography on oligo(dT)-cellulose columns, is used for eukaryotic mRNA extraction, instead of using oligo(dT)-cellulose we can also use

TLS Online TPP Program

#Question id: 18074

#SCPH06 I Botany

Affinity chromatography on oligo(dT)-cellulose columns, is used for eukaryotic mRNA extraction, instead of using oligo(dT)-cellulose we can also use

TLS Online TPP Program

#Question id: 18075

#SCPH01 Biochemistry

At high salt concentrations, the mRNA containing poly(A) tails binds to the complementary oligo(dT) molecules of the affinity column, and so, mRNA will be retained; all other RNA molecules can be washed through the column by further high salt solution. finally, the bound mRNA can be eluted by using

TLS Online TPP Program

#Question id: 18075

#I Life Science/ Life Sciences Group – I-V

At high salt concentrations, the mRNA containing poly(A) tails binds to the complementary oligo(dT) molecules of the affinity column, and so, mRNA will be retained; all other RNA molecules can be washed through the column by further high salt solution. finally, the bound mRNA can be eluted by using

TLS Online TPP Program

#Question id: 18075

#SCPH05 I Biotechnology

At high salt concentrations, the mRNA containing poly(A) tails binds to the complementary oligo(dT) molecules of the affinity column, and so, mRNA will be retained; all other RNA molecules can be washed through the column by further high salt solution. finally, the bound mRNA can be eluted by using

TLS Online TPP Program

#Question id: 18075

#SCPH06 I Botany

At high salt concentrations, the mRNA containing poly(A) tails binds to the complementary oligo(dT) molecules of the affinity column, and so, mRNA will be retained; all other RNA molecules can be washed through the column by further high salt solution. finally, the bound mRNA can be eluted by using