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#Question id: 13092
#SCPH01 Biochemistry
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?
TLS Online TPP Program
#Question id: 342
#I Life Science/ Life Sciences Group – I-V
In a single molecule of water, two hydrogen atoms are bonded to a single oxygen atom by ________.
TLS Online TPP Program
#Question id: 23148
#SCPH06 I Botany
There are two proteins A and B to be visualized under fluorescence microscopy. Both of them are excited with same wavelength of light but A emits longer wavelength. Hence we can conclude that
1. A is denser molecule than B
2. B is denser molecule than A
3. A is lighter molecule than B
4. B is lighter molecule than A
Which of the following option is incorrect?
TLS Online TPP Program
#Question id: 745
#I Life Science/ Life Sciences Group – I-V
The structure of RNA differs from that of DNA, as RNA contains:
TLS Online TPP Program
#Question id: 2658
#SCPH06 I Botany
This condition in lac operon facilitates the condition of lac genes being transcribed at high levels